Annals of the XIII International Fungal Biology Conference (IFBC) & IV International Symposium on Fungal Stress (ISFUS)

ASPHYXIATION OF METARHIZIUM ROBERTSII DURING MYCELIAL GROWTH PRODUCES CONIDIA WITH INCREASED STRESS TOLERANCE VIA INCREASED EXPRESSION OF STRESS-RELATED GENES

Published in 13/12/2022 - ISBN: 978-85-5722-449-0

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Paper Title
ASPHYXIATION OF METARHIZIUM ROBERTSII DURING MYCELIAL GROWTH PRODUCES CONIDIA WITH INCREASED STRESS TOLERANCE VIA INCREASED EXPRESSION OF STRESS-RELATED GENES
Authors
  • Albery Martins
  • Drauzio Eduardo Naretto Rangel
  • Breno pupin
  • Nicolás Pedrini
Modality
Resumo
Subject area
ISFUS - Fungal stress in agriculture
Publishing Date
13/12/2022
Country of Publishing
Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/isfus2021/528218-asphyxiation-of-metarhizium-robertsii-during-mycelial-growth-produces-conidia-with-increased-stress-tolerance-via
ISBN
978-85-5722-449-0
Keywords
entomopathogenic fungi, cross-resistance, hypoxia, transient anoxia, normoxia, adaptation, nutritional stress, UV-B radiation, heat stress, oxidative stress, osmotic stress, genotoxic stress
Summary
Little is known about the impact of hypoxia and anoxia during mycelial growth on tolerance to different stress conditions of developing fungal conidia. Conidia of the insect-pathogenic fungus Metarhizium robertsii were produced on potato dextrose agar (PDA) medium under normoxia (control = normal oxygen concentrations), continuous hypoxia, and transient anoxia, as well as minimal medium under normoxia. The tolerance of the conidia produced under these different conditions was evaluated in relation to wet-heat (heat stress), menadione (oxidative stress), potassium chloride (osmotic stress), UV radiation, and 4-nitroquinoline-1-oxide (=4-NQO genotoxic stress). Growth under hypoxic condition induced higher conidial tolerance of M. robertsii to menadione, KCl, and UV radiation. Transient anoxic condition induced higher conidial tolerance to KCl and UV radiation. Nutritional stress (i.e., minimal medium) induced higher conidial tolerance to heat, menadione, KCl, and UV radiation. The gene hsp30 and hsp101 encoding a heat shock protein was over expressed under anoxic condition. In conclusion, growth under hypoxia and anoxia produced conidia with higher stress tolerance than conidia produced in normoxic condition. The nutritive stress generated by minimal medium, however, induced a much higher stress tolerance. This condition also caused the highest level of gene expression in the hsp30 and hsp101 genes. Allowing to conclude that there has been a greater adaptation to stress of the conidia produced under nutritive stress, hypoxia and anoxia. POSTER PRESENTATION
Title of the Event
IV International Symposium on Fungal Stress - ISFUS & International Fungal Biology Conference - IFBC
City of the Event
São José dos Campos
Title of the Proceedings of the event
Annals of the XIII International Fungal Biology Conference (IFBC) & IV International Symposium on Fungal Stress (ISFUS)
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

MARTINS, Albery et al.. ASPHYXIATION OF METARHIZIUM ROBERTSII DURING MYCELIAL GROWTH PRODUCES CONIDIA WITH INCREASED STRESS TOLERANCE VIA INCREASED EXPRESSION OF STRESS-RELATED GENES.. In: Annals of the XIII International Fungal Biology Conference (IFBC) & IV International Symposium on Fungal Stress (ISFUS). Anais...São José dos Campos(SP) Hotel Nacional Inn, 2022. Available in: https//www.even3.com.br/anais/ISFUS2021/528218-ASPHYXIATION-OF-METARHIZIUM-ROBERTSII-DURING-MYCELIAL-GROWTH-PRODUCES-CONIDIA-WITH-INCREASED-STRESS-TOLERANCE-VIA. Access in: 26/04/2025

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