ACETATE ATTENUATES DSS-INDUCED COLITIS IN MICE THROUGH FFAR2 SIGNALING

Sarah de Oliveira; José Luís Fachi; Lais Passariello Pral; Mariane Font Fernandes; Arilson B. S. P. Gomes; Helder Carvalho de Assis; JEFFERSON ANTONIO DE CASTRO DOS SANTOS; VALQUIRIA APARECIDA MATHEUS; Prof. Dr. Marco Aurélio Vinolo

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Trabalho

Título do Trabalho

ACETATE ATTENUATES DSS-INDUCED COLITIS IN MICE THROUGH FFAR2 SIGNALING

Autores

Sarah de Oliveira
José Luís Fachi
Lais Passariello Pral
Mariane Font Fernandes
Arilson B. S. P. Gomes
Helder Carvalho de Assis
JEFFERSON ANTONIO DE CASTRO DOS SANTOS
VALQUIRIA APARECIDA MATHEUS
Prof. Dr. Marco Aurélio Vinolo

Modalidade

Apresentação de Pôster

Área temática

Imunologia

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609467-acetate-attenuates-dss-induced-colitis-in-mice-through-ffar2-signaling

ISBN

978-85-5722-785-9

Palavras-Chave

Inflammation, neutrophils, SCFAs, FFAR2.

Resumo

Introduction: short-chain fatty acids (SCFA) are molecules produced by the intestinal microbiota through the fermentation of dietary non-digestible carbohydrates. These molecules play a role on the microbiota-host interaction and modulate many biological functions in different cell types, such as activation and chemotaxis of neutrophils, in part through activation of G protein-coupled receptors, such as FFAR2. Studies on the relation of the FFAR2 receptor and intestinal inflammation indicate that this receptor play an important role in the development of inflammatory diseases and its crucial for mucosal immunity. However, the mechanisms behind this effect and the target cells remains unclear. Here, we analyzed the role of FFAR2 expressed in neutrophils in intestinal inflammation. Methods and Results: we used 8-week-old male mice with conditional deletion of FFAR2 in neutrophils, treated or not with 150 mM acetate during 12 days and in the last 6 days they received 2.5% dextran sodium sulfate (DSS) supplementation in drinking water for colitis induction. We observed that acetate ameliorates the clinical development of intestinal inflammation. Acetate-treated mice also showed an increase of neutrophils population in bone marrow and blood, indicating an effect on these cells’ production/differentiation. Using conditional knockouts, we noticed the phenotype was partially dependent on FFAR2, since the conditional deletion of this receptor impacted the observed protective effect. Conclusion: thus, we preliminarily conclude that acetate regulates the production of neutrophils in the bone marrow and its maintenance on peripheric tissues, and FFAR2 activation by this SCFA in neutrophils participate in the protective effect during a colitis model.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

OLIVEIRA, Sarah de et al.. ACETATE ATTENUATES DSS-INDUCED COLITIS IN MICE THROUGH FFAR2 SIGNALING.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609467-ACETATE-ATTENUATES-DSS-INDUCED-COLITIS-IN-MICE-THROUGH-FFAR2-SIGNALING. Acesso em: 22/12/2024