ROLE OF THE ARABIDOPSIS THALIANA ATBZIP63 TRANSCRIPTION FACTOR STABILITY IN ENERGY MANAGEMENT

Pamela Tavares Carlson; João Guilherme Portugal Vieira; Raphael de Araújo Campos; Thyelen Engel de Jesus; Luis Felipe Correa da Silva; Nubia Barbosa Eloy; Cleverson Carlos Matiolli; Michel Vincentz

Todos os Trabalhos

Trabalho

Título do Trabalho

ROLE OF THE ARABIDOPSIS THALIANA ATBZIP63 TRANSCRIPTION FACTOR STABILITY IN ENERGY MANAGEMENT

Autores

Pamela Tavares Carlson
João Guilherme Portugal Vieira
Raphael de Araújo Campos
Thyelen Engel de Jesus
Luis Felipe Correa da Silva
Nubia Barbosa Eloy
Cleverson Carlos Matiolli
Michel Vincentz

Modalidade

Apresentação de Pôster

Área temática

Genética Vegetal e Melhoramento

Data de Publicação

09/06/2023

País da Publicação

Brasil

Idioma da Publicação

Inglês

Página do Trabalho

https://www.even3.com.br/anais/v-gbmeeting/609552-role-of-the-arabidopsis-thaliana-atbzip63-transcription-factor-stability-in-energy-management

ISBN

978-85-5722-785-9

Palavras-Chave

Arabidopsis thaliana, bZIP63, energy, gene expression, protein stability

Resumo

Plants have evolved adaptive mechanisms dedicated to adjust their growth and development according to nutrients and energy availability. The perception of energetic limitations by SnRK1 results in the activation of the transcription regulation factor bZIP63 that, along with its dimerization partners bZIP1 and bZIP53, and the circadian clock, promote a gene expression program dedicated in mitigating the adverse effects of lack of energy. Taking the central role of bZIP63 in the adjustment of the energetic balance into account, it is essential to understand how this gene’s expression is regulated. We have shown that the bZIP63 mRNA accumulation profile throughout the circadian clock is defined by the interaction between the circadian clock and sugars. On the other hand, the activity and stability of the bZIP63 protein are unknown aspects. This project’s main objective is to evaluate the importance of the regulation of the bZIP63 protein over the format of the regulation network related to bZIP63 throughout the diel cycle. Most specifically, our main objectives are to: 1- evaluate the stability and activity of the bZIP63 protein through the diel cycle; 2- evaluate the control of bZIP63’s stability mediated by the D-box and its interaction with the ubiquitin signaling complex’s subunit, APC10. Null mutants for bZIP63 had been complemented with a fusion between bZIP63 cDNA, a hemagglutinin (HA) TAG and the Green Fluorescent protein (GFP) which is transcribed from the CaMV 35S promoter (35S:HA:cDNAbZIP63:GFP). Complementation was validated by the expression of the fusion protein HA:bZIP63:GFP and the reestablishment of bZIP63 target genes (BTP/POZ; ASN1; DIN10; PRR7; PRODH). Although these complementation genotypes exhibited consistent developmental and growth peculiarities as compared to the wild type, they will be used to analyze bZIP63 stability along the diel cycle. The interaction between bZIP63 and APC/C’s complex subunits was tested through a yeast-two-hybrid (Y2H) system. Preliminary results showed an interaction between the SAMBA subunit of the APC/C complex.Considering the potential interaction between SAMBA and the bZIP63, we can now test the interaction between other APC/C subunits and bZIP63, and, through mutating the D-box in bZIP63, confirm that the interaction between APC10 and bZIP63 is done through it.

Título do Evento: V GBMeeting
Cidade do Evento: Campinas
Título dos Anais do Evento: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP
Nome da Editora: Even3
Meio de Divulgação: Meio Digital

Como citar

CARLSON, Pamela Tavares et al.. ROLE OF THE ARABIDOPSIS THALIANA ATBZIP63 TRANSCRIPTION FACTOR STABILITY IN ENERGY MANAGEMENT.. In: Anais do GBMeeting: Encontro Anual da Pós Graduação em Genética e Biologia Molecular da UNICAMP. Anais...Campinas(SP) Unicamp, 2023. Disponível em: https//www.even3.com.br/anais/v-gbmeeting/609552-ROLE-OF-THE-ARABIDOPSIS-THALIANA-ATBZIP63-TRANSCRIPTION-FACTOR-STABILITY-IN-ENERGY-MANAGEMENT. Acesso em: 21/12/2024