Anais do XV LASEAC (Latin American Symposium on Environmental Analytical Chemistry) & X ENQAmb (Encontro Nacional de Química Ambiental)

SYNTHESIS OF SPENT COFFEE GROUNDS CARBON DOT (SCG-CD): BIOLOGICAL AND ECOTOXICOLOGICAL EVALUATION

Publicado em 15/04/2024 - ISBN: 978-65-272-0404-6

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Título do Trabalho
SYNTHESIS OF SPENT COFFEE GROUNDS CARBON DOT (SCG-CD): BIOLOGICAL AND ECOTOXICOLOGICAL EVALUATION
Autores
  • Luana Caroline de Oliveira Lima
  • Janaina Domingas Alves
  • Amanda Maria Siqueira Moreira
  • Letícia Regina de Carvalho Cunha
  • Paulo Henrique de Almeida Campos Junior
  • Érika Cristina Jorge
  • Helio Batista dos Santos
  • Ralph Gruppi Thomé
  • Marco Antonio Schiavon
Modalidade
Resumo
Área temática
Ecotoxicologia
Data de Publicação
15/04/2024
País da Publicação
Brasil
Idioma da Publicação
Português
Página do Trabalho
https://www.even3.com.br/anais/xv-laseac-x-enqamb-2024i/786686-synthesis-of-spent-coffee-grounds-carbon-dot-(scg-cd)--biological-and-ecotoxicological-evaluation
ISBN
978-65-272-0404-6
Palavras-Chave
carbon dot, spent coffee ground, in vivo and invitro toxicity, ecotoxicity
Resumo
As the third largest commodity in the world, coffee is also a product that generates the most industrial waste, both in its production process – powder or soluble coffee – and its consumption – spent coffee grounds (SCG). In addition, these SCGs are composed of a huge amount of organic compounds which can cause the contamination of water resources. As a solution for this problem, the synthesis of Carbon Dot (CD) can be turning promisors among other paths. CDs are nanomaterials with sp²/sp³ configuration, basically based on carbon, oxygen, and hydrogen in size distribution between 2 -10 nm. So, the present work used SCG to synthesize CD through the pyrolysis technique under oxidizing air. Thermogravimetric analysis (TGA), fluorescence spectroscopy (PL), and design of experiment (DoE) were used to map the synthesis conditions and the method, which found out the best synthesis condition at 400°C and 3h (BC4). Related to optical aspects, BC4 showed a quantum yield efficiency (?=6,0%) on absorption and emission maximum wavelength of 334 nm and 430 nm (?exc= 310nm), respectively, and a bi-exponential lifetime decay (a_1 = 0,5, t_1 = 1,60 ns; a_2 = 0,48, t_2 = 5,44 ns). Photostability was also evaluated by the Britton-Robinson buffer solution (ph=2 to ph=12) and it showed an increase in the emission signal until ph=11. This can be related to the improvement of surface states on BC4. Regarding its composition and structure were observed the presence of Carbon Dot common bonds (C=C, C-OH, C=O) and leachable metals, which are typically found in the Coffee's nutritional composition. These metals were seen and confirmed by Energy Dispersive Spectroscopy and X-Ray. Exploring the BC4 toxicity issue in vitro and in vivo assays were performed. The MTT method was evaluated on fibroblast cells and showed no significative statistical difference (p<0.05) between the control and group 20 ug/mL in a 5°-day period. Besides, fluorescent microscopy analysis indicated the PC into the cell nucleus. An in-vivo test in mice was performed and has no significative statistical difference (p<0.05) observation between the control and the highest concentration (2000 mg/kg) in a 14°-day period. In response to the problem of SCG disposed of in an aquatic environment, the ecotoxic effect was conducted by the Fish Embryo Acute Toxicity (FET) on zebrafish which had a statistical difference (p<0.05) between the control and the 500 ug/mL group. As shown, the results indicate that SCG is a promising source for the synthesis of CDs with an affinity for the cell nucleus and low toxicity for mammals, however, remains necessary to rethink regarding to the environment concerning.
Título do Evento
XV LASEAC - X ENQAmb 2024.
Cidade do Evento
Ouro Preto
Título dos Anais do Evento
Anais do XV LASEAC (Latin American Symposium on Environmental Analytical Chemistry) & X ENQAmb (Encontro Nacional de Química Ambiental)
Nome da Editora
Even3
Meio de Divulgação
Meio Digital

Como citar

LIMA, Luana Caroline de Oliveira et al.. SYNTHESIS OF SPENT COFFEE GROUNDS CARBON DOT (SCG-CD): BIOLOGICAL AND ECOTOXICOLOGICAL EVALUATION.. In: . Disponível em: https//www.even3.com.br/anais/xv-laseac-x-enqamb-2024i/786686-SYNTHESIS-OF-SPENT-COFFEE-GROUNDS-CARBON-DOT-(SCG-CD)--BIOLOGICAL-AND-ECOTOXICOLOGICAL-EVALUATION. Acesso em: 24/04/2025

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