X-Meeting presentations

END OF THE BEGINNING: M. INCOGNITA TELOMERES ARE ONLY AT ONE SIDE OF THE CHROMOSOMES.

Published in 08/11/2023 - ISBN: 978-65-272-0061-1

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Paper Title
END OF THE BEGINNING: M. INCOGNITA TELOMERES ARE ONLY AT ONE SIDE OF THE CHROMOSOMES.
Authors
  • Ana Mota
  • Georgios D Koutsovoulos
  • Laetitia Perfus-Barbeoch
  • Evelin Despot-Slade
  • Karine Labadie
  • Jean-Marc Aury
  • Karine Robbe-Sermesant
  • Marc Bailly-Bechet
  • Caroline Belser
  • Arthur Péré
  • Corinne Rancurel
  • Djampa Kozlowski
  • Rahim Hassanaly-Goulamhoussen
  • Martine da Rocha
  • Benjamin Noël
  • Nevenka Mestrovic Radan
  • Patrick Wincker
  • Etienne G J Danchin
Modality
Poster
Subject area
DNA and Genomics
Publishing Date
08/11/2023
Country of Publishing
Brazil | Brasil
Language of Publishing
Inglês
Paper Page
https://www.even3.com.br/anais/xmeeting2023/639825-end-of-the-beginning--m-incognita-telomeres-are-only-at-one-side-of-the-chromosomes
ISBN
978-65-272-0061-1
Keywords
Telomeres, Root-knot nematodes, ONT, assembly
Summary
Assembling the genomes of polyploid hybrid species is a challenge, due to variable degrees of divergence between the genome copies. Unlike in polyploid hybrids, classical sexually-reproducing species have diploid genomes with two highly similar chromosome sets inherited from their parents. However, for the root-knot nematode Meloidogyne incognita, it is hypothesized that three chromosome sets with various degrees of sequence divergence compose its triploid genome (A'A"B). We have generated genomic data using long-reads from ONT technology for M. incognita. Using NECAT we assembled the genome in 291 contigs which we further polished with both ONT long reads and Illumina short reads. This allowed resolving and assembling highly repetitive regions of the M. incognita genome for the first time. Thanks to this new assembly, we could investigate the telomere sequences of M. incognita. Against all odds, we could find neither the telomerase genes nor the canonical telomeric repeat sequence of C. elegans. However, at the end of some assembled contigs, we found a composite G-rich repeat. We compared this new repetitive sequence against all nematode species with a sequenced genome, and apart from partial matches in closely related Meloidogyne species, the repeat was absent from the rest of the nematodes. Using fluorescent in-situ hybridization we confirmed the repeat had a telomeric localization but only at one extremity of the chromosomes. The discovery of a new kind of telomeric repeat in these species highlights the evolutionary diversity of chromosome protection systems despite their central roles and opens new perspectives towards the development of more specific control methods against these pests.
Title of the Event
X-Meeting / BSB 2023
City of the Event
Curitiba
Title of the Proceedings of the event
X-Meeting presentations
Name of the Publisher
Even3
Means of Dissemination
Meio Digital

How to cite

MOTA, Ana et al.. END OF THE BEGINNING: M. INCOGNITA TELOMERES ARE ONLY AT ONE SIDE OF THE CHROMOSOMES... In: X-Meeting presentations. Anais...Curitiba(PR) Campus da indústria, 2023. Available in: https//www.even3.com.br/anais/xmeeting2023/639825-END-OF-THE-BEGINNING--M-INCOGNITA-TELOMERES-ARE-ONLY-AT-ONE-SIDE-OF-THE-CHROMOSOMES. Access in: 05/02/2025

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